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Channel: PCR, qPCR and qRT-PCR – Bitesize Bio
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11 qPCR Papers Every Researcher Should Know

Whether you're generating, analyzing, or reviewing qPCR data you need to understand how it works and best practices. That's why we've pulled together our top qPCR papers every researcher should read.

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4 Easy Steps to Analyze Your qPCR Data Using Double Delta Ct Analysis

New to qPCR? Here's a quick summary of one of the two most common analysis methods - double delta Ct analysis.

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A Step-by-Step Guide to Designing qPCR Primers

qPCR primer design is a bit of science, a bit of magic, and a little bit of luck. Here's the science to help you design the best primers for your experiments.

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What is PCR? The Beginner’s Guide

If you need to copy, sequence, or quantify DNA, you need to know about PCR. Read our guide to the PCR process, and discover tips to help you avoid the most common PCR pitfalls.

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The qPCR Standard Curve: The Key to Good qPCR Data

Can't be bothered doing a qPCR standard curve? Find out why it's important to getting great results and how to do it.

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What is Touchdown PCR? A Primer and 5 Easy Tips For Success

Got non-specific PCR amplification? You need touchdown PCR! Discover what it is, how it works, and get 5 top tips for performing touchdown PCR.

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Methods for Relative Quantification of qPCR Data: Yes, There is More Than One

Want to know the best way to perform relative quantification for your qPCR data? Read on.

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How to Build Your Own PCR Machine

You can build your own PCR machine using tools and equipment you can get easily and without breaking the bank. Well, what are you waiting for?

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Overhang PCR: Add Missing DNA Sequences Using Primers

Need to add extra nucleotides to your plasmid or other DNA sample? Here's how to use overhang PCR to easily add extra bases using primers.

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What Is a Cq (Ct) Value?

Don't be confused by Ct values! We'll guide you through what they are, how to calculate them, and troubleshooting issues.

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